ADVANCES IN GENETICALLY MODIFIED ANIMALS
ISBN: 978-1-83926-763-5
Year: 2022
Binding: HB
Price: US $ 295.00
About Book
Genetically modified animals embody an innovative technology that is transforming world through biomedical, environmental and food applications. Genetic modification of an animal involves altering its genetic material by adding, changing or removing certain DNA sequences in a way that does not occur naturally. With advances in the field, new technology emerged that did not necessarily require transgenesis, recent applications allow for the creation of genetically engineered animals via the deletion of genes, or the manipulation of genes already present. To reflect this progress and to include those animals that are not strictly transgenic, the umbrella term “genetically engineered” has been adopted into the guidelines. It also brings with it concerns over intellectual property, and patenting of created animals and the techniques used to create them. Genetic engineering technology has numerous applications involving companion, wild, and farm animals, and animal models used in scientific research. This book is a valuable compilation of topics, ranging from the basic to the most complex theories and principles in the field. It focuses on various aspect of genetic modification of domestic animals for agriculture and biomedical applications. It is designed to provide understanding of issues such as genetic diversity and evolution of marine animals isolated in marine lakes, genetics in domestic animal reproduction and recent advances in genome editing-based gene modification in pigs. It also focuses on genetic engineering and cloning, hox genes and toxins from venomous animals. Some of the diverse topics covered in this book address the varied branches that fall under this category. For all those who are interested in animal genetics and breeding, this book can prove to be an essential guide.
Preface............................................................................................................v
Chapter 1 Recent Advance in Genome Editing-Based Gene Modification in Pigs.................................................................................................................1
Abstract...................................................................................................1
1. Introduction............................................................................................2
2. Background of second-generation genome editing........................4
3. History of GE in pigs...........................................................................5
4. Delivery method..................................................................................12
5. Other techniques and factors affecting efficacy of the genome
editing system......................................................................................23
6. Conclusion............................................................................................27
Acknowledgements.............................................................................27
Conflicts of interest.............................................................................28
Author contributions..........................................................................28
Chapter 2 Genetics in Domestic Animal Reproduction ..................................45
Abstract.................................................................................................45
1. Genetics during germ cell produCtion...........................................45
2. Genetics during fertilization.............................................................47
3. Genetics of eutherian sex determination and dose
compensation.......................................................................................48
4. Genomic imprinting............................................................................49
5. Genetics during preimplantative eutherian embryo
development.........................................................................................50
6. Genetics during mammalian placentation and fetal
development.........................................................................................51
7. Genetic alterations caused by assisted reproduction
techniques.............................................................................................52
8. Genetics in poultry reproduction....................................................53
9. Genetics in fish reproduction...........................................................54
10. Further perspectives of genetics in domestic animal
reproduction.........................................................................................54
Chapter 3 Toxins from Venomous Animals: Gene Cloning, Protein Expression
and Biotechnological Applications.......................................................58
1. Introduction..........................................................................................58
2. Biotechnological and pharmacological applications of snake
venom toxins........................................................................................60
3. Biotechnological and pharmacological applications of scorpion
venom toxins........................................................................................65
4. Biotechnological and pharmacological applications of spider
venom toxins........................................................................................69
5. Biotechnological and pharmacological applications of toad and
frog toxins............................................................................................72
6. Biotechnological and pharmacological applications of bee and
wasp toxins..........................................................................................75
7. Biotechnological and pharmacological applications of ant,
centipede and caterpillar venom toxins.........................................80
8. Conclusion............................................................................................88
Acknowledgements.............................................................................89
Chapter 4 Genetic Engineering and Cloning: Focus on Animal
Biotechnology...........................................................................................116
1. Introduction........................................................................................116
2. Genetic engineering: Timeline........................................................119
3. Cutting and pasting the DNA........................................................121
4. DNA cloning......................................................................................124
5. Isolation, sequencing and synthesis DNA...................................124
6. Cloning vectors..................................................................................133
7. Practical application of genetic Engineering and cloning:
From transgenic animal models until cloning animal..............143
8. Future Prospects — Genetic engineering and cloning: A dream
or a nightmare?.................................................................................152
Acknowledgements .........................................................................155
Chapter 5 Hox Genes: Master Regulators of the Animal Bodyplan............161
1. Introduction ......................................................................................161
2. Discovery and cloning of the Hox genes, their role and regulation
in Drosophila ......................................................................................... 161
3. Hox clustering and colinearity: The key property ....................163
4. The evolution of Hox genes in different taxa, including
vertebrates .........................................................................................166
5. The mechanism of Hox collinearity .............................................168
6. Hox function in vertebrates ...........................................................172
7. Conclusions .......................................................................................179
8. Acknowledgements .........................................................................180
Chapter 6 Genetic Diversity and Evolution of Marine Animals Isolated in
Marine Lakes ..........................................................................................187
1. Introduction........................................................................................187
2. Genetic diversity and evolution in coral reefs...........................189
3. Genetic diversity and evolution in the marine lakes of the Palau
Islands.................................................................................................192
4. Acknowledgments ...........................................................................217
Chapter 7 Genetic Modification of Domestic Animals for Agriculture and
Biomedical Applications ......................................................................224
1. Introduction ......................................................................................224
2. Recent progress and applications of trans-genic domestic
animals ...............................................................................................225
3. Technical aspects of cell-based transgenesis by SCNT ............230
4. Improvement of the efficiency of cell-based transgenesis via
SCNT ..................................................................................................238
5. Summary ...........................................................................................245
Citation......................................................................................................265
Index...........................................................................................................267
